4ARM-PEG-Silane 四臂PEG硅烷
基本信息：
中文名称：四臂聚乙二醇硅烷(4ARM-PEG-Silane)
中文别名：四臂PEG硅烷
英文名称：4ARM-PEG-Silane
CAS：-
分子量：2000,5000，10000,20000
纯度：95%以上
应用简介：
silanePEG (PEG-Si) is a surface reactive PEG derivative that can be used to modifyglass, silica and other surfaces via the reaction between hydroxyl group andethoxyl/methoxyl silane. Pegylation can greatly suppress the non-specificbinding of charged molecules to the modified surfaces. And they have wideapplications for medica device, biomems or biocompatible material development.
ReactionProcedures:
Generally,silane PEG is first dissolved in a mixture of ethanol/water solution, thenallow the solution to contact with oxidized glass or silica surface for 30 minto 2 hours. Under this condition, silane PEG should bind to the materialcovalently.
MaterialsRequired:
Pegylationsolution: Ethanol/water (w/w, 95%/5%);
SilanePEG stock solution, 10~50 mg/mL in pegylation solution, prepare in fresh.
Washingsolution: Distilled water.
ReactionSteps:
1.Dissolve targeted materials in Pegylation buffer.
2. Addsilane PEG stock solution to the targeted conjugation materials with the finalconcentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG neededfor optimal conjugation;
3.Allow mixture agitates at room temperature for 30 min at room temperature or 2hours at 4 -20℃.
4. Washout unreacted materials either by distilled water.
物理性质：
状态：液体或者固体。
溶解性：溶于DMSO,DMF,DCM，溶于水。
保持条件：-20℃长期保存，避光，干燥。
注意事项：取用一定要干燥，避免频繁的溶解和冻干。
技术指标：
纯度（PEG）：95%以上
取代率（Silane）：95%以上
分散系数（PDI）：小于等于1.05
分析：COA、GPC、NMR、MALDI-TOF