方差分析的R语言实现包括以下部分:
数据导入
数据清洗
ANOVA计算
结果解析
ANOVA评估
knitr::opts_chunk$set(message = FALSE, warning = FALSE)
options(stringsAsFactors = F)
options(future.globals.maxSize = 1000 * 1024^2)
install.packages(c("ggplot2", "ggpubr", "tidyverse"))
library(tidyverse) # 数据预处理R包
library(readxl) # 读取xlsx数据R包
library(ggpubr) # 画图R包
data <- data.frame(D = c(rep("A", 4), rep("B", 4), rep("C", 4), rep("D", 4), rep("E", 4), rep("F", 4)),
RR = c(80,83,83,85,75,75,79,79,74,73,76,77,67,72,74,74,62,62,67,69,60,61,64,66))
write.table(data, file = "data.txt", sep = "\t", quote = F, row.names = F)
xlsx::write.xlsx(data, file = "data.xlsx", row.names = F)
data <- read.table("data.txt", header = T)
data <- read_xlsx("data.xlsx", sheet = 1)
data_drop <- data %>%
dplyr::filter(D != "A")#%>%
#dplyr::mutate(Test = "test")
head(data_drop)
data %>%
group_by(D) %>%
summarise(N=n(),
Means=mean(RR),
SS=sum((RR - Means)^2),
SD=sd(RR),
SEM=SD/N^.5)
ggboxplot(data_drop,
x = "D",
y = "RR",
color = "D",
ylab = "RR", xlab = "D")
one-way ANOVAs: 使用aov函数运行单因素方差分析 (公式是:Y是检验变量,X是分组变量);
再使用summary函数获取单因素方差分析的结果。
# Y=RR; X=D
one.way <- aov(RR ~ D, data = data_drop)
summary(one.way)
结果解析:
Residuals
是模型的残差,可以理解为截距;
Df
列显示了自变量的自由度(变量中的水平数减1)和残差的自由度(观察总数减1和自变量中的水平数减1);
Sum Sq
列显示平方和(即组均值与总体均值之间的总变化)。;
Mean Sq
列是平方和的平均值,通过将平方和除以每个参数的自由度来计算;
F value
列是F检验的检验统计量。这是每个自变量的均方除以残差的均方。F值越大,自变量引起的变化越有可能是真实的,而不是偶然的;
Pr(>F)
列是F统计量的p值。这表明,如果组均值之间没有差异的原假设成立,那么从检验中计算出的F值发生的概率大小。
另一种方法:t-test仅仅适合2组比较,因此需要筛选
data_ttest <- data_drop %>%
dplyr::filter(D %in% c("B", "C")) #%>%
#dplyr::filter(RR != 77)
# data_test_filter <- filter(data_drop, D %in% c("B"))
# data_test_filter2 <- filter(data_test_filter, RR != 77)
t.test(RR ~ D, data = data_ttest)
ANOVA结果仅仅揭示多个组间的差异结果,具体到哪两个组内部差异还需要做后置检验
后置检验通常采用TukeyHD
函数
TukeyHSD(one.way)
该结果给出每个两组之间的结果;
diff
: 两组的均值之差;
Lwr, upr
: 95%置信区间的下限和上限(默认值) ;
P adj
: 多次比较调整后的P值。
plot(one.way, 2)
shapiro.test(x = residuals(object = one.way))
结果显示:残差不显著也即是表明残差服从正态分布,可以采用ANOVA分析方法判断RR在D分组的分布水平。
library(car)
leveneTest(RR ~ D, data = data_drop, center = mean)
bartlett.test(RR ~ D, data = data_drop)
devtools::session_info()
─ Session info ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
setting value
version R version 4.3.1 (2023-06-16)
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system x86_64, darwin20
ui RStudio
language (EN)
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tz Asia/Shanghai
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rstudio 2023.09.0+463 Desert Sunflower (desktop)
pandoc 3.1.1 @ /Applications/RStudio.app/Contents/Resources/app/quarto/bin/tools/ (via rmarkdown)
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