Kamiya丨Kamiya艾美捷抗FLAG多克隆说明书

Kamiya艾美捷抗FLAG多克隆化学性质：

程序：用FLAG肽免疫家兔与KLH偶联。多次免疫后在弗氏佐剂中收集血清使用固定在固相上的肽。

规范：

使用氨基末端分析抗体Met FLAG BAP、氨基末端FLAG-BAP和羧基末端FLAG-BAP融合蛋白和Invitrogen Posite R900-40。

宿主：兔子

格式：液体PBS中的亲和纯化抗体，pH 7.2含0.1%叠-氮-化钠。

Kamiya艾美捷抗FLAG多克隆储存和稳定性：

在+4°C下储存。

应用和建议的稀释液：

ELISA：在1:5000-1:20000时使用。

蛋白质印迹：使用1:5000-1:100000。

特定应用的最佳稀释度应由研究人员确定。

仅用于体外研究。不适用于诊断或人类。

Kamiya艾美捷抗FLAG多克隆英文说明：

Procedure:

Rabbits were immunized with the FLAG peptide conjugated to KLH. After multiple immunizations

in Freund’s adjuvant, serum was collected and the antibodies were affinity-purified using the

peptide immobilized on a solid phase.

Specification:

Antibody was analyzed using amino-terminal Met-FLAG-BAP, amino-terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40.

Host: Rabbit

Format:

Liquid. Affinitiy-purified antibody in PBS, pH 7.2 with 0.1% sodium azide.

Storage and Stability:

Store at +4°C.

Applications and Suggested Dilutions:

ELISA: Use at 1:5,000-1:20,000.

Western blot: Use at 1:5,000-1:10,000.

The optimal dilution for a specific application

should be determined by the researcher.

Limitations:

For in vitro research use only. Not for use in

diagnostics or in humans.