Kamiya丨Kamiya艾美捷抗-C-Myc，多克隆说明书

Kamiya艾美捷抗-C-Myc，多克隆化学性质：

程序：鸡用C-Myc免疫与KLH缀合的肽。多次之后用弗氏佐剂免疫收集。使用固定在固体上的肽对抗体进行免疫亲和纯化阶段

规范：

ELISA：抗体特异性通过针对与BSA缀合的肽的ELISA。A.1： 38000稀释的抗体得到OD=

1.0，在15分钟反应中使用HRP-缀合的山羊抗鸡IgY（IgG）1： 10000和TMB作为基底。适当的进行特异性对照。

宿主：鸡

格式：液体PBS中的亲和纯化抗体，pH 7.2含0.1%叠-氮-化钠。

Kamiya艾美捷抗-C-Myc，多克隆储存和稳定性：

在+4°C下储存。

应用和建议的稀释液：酶联免疫吸附试验

蛋白质印迹：约1:5000时使用特定应用的最佳稀释度应由研究人员确定。

仅用于体外研究。不适用于诊断或人类。

Kamiya艾美捷抗-C-Myc，多克隆英文说明：

Procedure:

Chickens were immunized with the C-Myc

peptide conjugated with KLH. After multiple

immunizations in Freund’s adjuvant, eggs were

collected. Antibodies were immunoaffinitypurified using the peptide immobilized on a solid

phase.

Specification:

ELISA: Antibody specificity was verified by

ELISA against the peptide conjugated to BSA. A

1:38,000 dilution of the antibody gave an OD =

1.0 in a 15 minute reaction using HRPconjugated Goat anti-Chicken IgY (IgG) at

1:10,000 and TMB as the substrate. Appropriate

specificity controls were run.

Host: Chicken

Format:

Liquid. Affinity-purified antibody in PBS, pH 7.2

with 0.1% sodium azide.

Storage and Stability:

Store at +4°C.

Applications and Suggested Dilutions:

ELISA

Western blot: Use at ~1:5,000

The optimal dilution for a specific application

should be determined by the researcher.

Limitations:

For in vitro research use only. Not for use in

diagnostics or in humans.