中英文说明书丨Abbkine通用型免疫荧光工具箱（抗小鼠Dylight 488）

Based on the pain points of traditional IF experiment, such as tedious and time-consuming operation, various reagent supplies, and poor stability and reliability of experimental results, Abbkine developed a universal immunofluorescence (IF) toolbox. It can be applied to immunofluorescence detection of tissue and cell samples.

Kit components：

• Immunostaining Permeabilization Buffer-100 mL

• EDTA Antigen Retrieval Solution pH 8.0 (20×)-100 mL

• PBS (20×)-100 mL

• Goat Serum Blocking Buffer-20 mL

• Antibody Wash Buffer (20×)-25 mL

• Antibody Dilution Buffer-50 mL

• DyLight 488, Goat Anti-Mouse IgG-100 μL

• DAPI (500×)-100 μL

• SuperKine™ Enhanced Antifade Mounting Medium-10 mL

Features&Advantages

• ① Easy operation, complete components, no need to worry about complex reagents, and the experiment can be started by opening the box;

• ② Provide optimized antibody diluent and SuperKine ™ Enhanced anti fluorescence quenching agent, with strong anti quenching effect, maintains excellent fluorescence intensity;

• ③ Provide Dylight series secondary antibody with stronger fluorescence intensity and higher optical tolerance and specificity;

• ④ The goat anti mouse antibody with Dylight 488 tag can be used to detect the corresponding primary antibody from mice, and the color observed is bright green.

Abbkine通用型免疫荧光工具箱（抗小鼠Dylight 488）中文说明书：

背景：

免疫荧光技术（IF）是根据抗原抗体反应的原理，先将已知的抗原或抗体标记上荧光素，制成荧光抗体，再用这种荧光抗体（或抗原）作为探针检测组织或细胞内的相应抗原（或抗体）。

Abbkine通用型免疫荧光工具箱（抗小鼠Dylight 488）组分：

• 免疫染色透化缓冲液-100 mL

• EDTA 抗原修复溶液pH 8.0 (20×)-100 mL

• PBS (20×)-100 mL

• Goat Serum Blocking Buffer-20 mL

• Antibody Wash Buffer (20×)-25 mL

•抗体稀释缓冲液 - 50 mL

• DyLight 488，山羊抗小鼠 IgG-100 μL

• DAPI (500×)-100 μL

• SuperKine™ 增强型抗褪色封固剂 - 10 mL

Abbkine通用型免疫荧光工具箱（抗小鼠Dylight 488）特点：

• ①操作便捷，组分齐全，无需操心复杂试剂，开盒即可开始实验；

• ②提供优化的抗体稀释液和SuperKine™ 增强型抗荧光淬灭剂，抗淬灭效果强，维持J佳的荧光强度；

• ③提供Dylight系列二抗，具有更强的荧光强度和更高的光学耐受性及特异性；

• ④含有Dylight 488标记的山羊抗小鼠抗体，可以用于检测小鼠来源的相应一抗，观察到的颜色为鲜艳绿色。