中英文说明书丨艾美捷Actin聚合检测试剂盒的原理和应用

English name:Actin Polymerization Biochem Kit

supplier:Cytoskeleton

Product No.:BK003

Specifications:KIT30-100assays

The Actin Polymerization Biochem Kit™ is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. The enhanced fluorescence that occurs when pyrene G-actin (monomer) forms pyrene F-actin can be measured in a fluorimeter to follow polymerization over time. Also, by using preformed pyrene F-actin, it is possible to follow depolymerization. Both cell/tissue extracts and purified proteins can be added to the reaction mixture to identify their effect on actin polymerization. The components of the kit can also be used separately for other actin based assays such as a spin-down assays to detect F-actin binding proteins (see also BK001) or size exclusion chromatography to identify G-actin binding proteins. See the About Actin page for more information on assays testing actin binding proteins. While this kit comes with pyrene labeled skeletal muscle actin, it can also be used to study polymerization of other types of actin such as non-muscle actin (Cat. # APHL99) or cardiac actin (Cat. # AD99). Polymerization assays with these actins can be performed using a 10:1 ratio between the actin you want to study and the included pyrene actin

真核细胞的细胞骨架是与维持细胞形态结构和细胞运动有关的纤维网络，由微丝、微管和中间纤维组成。肌动蛋白单体（G-Actin）一个接一个连成一串肌动蛋白链，而微丝是由两股方向相同的肌动蛋白链以螺旋的形式组成的呈圆筒状的纤维。微丝能被组装和去组装。当单体上结合的是ATP时，就会有较高的相互亲和力，单体趋向于聚合成多聚体，就是组装。而当ATP水解成ADP后，单体亲和力就会下降，多聚体趋向解聚，即是去组装。那么肌动蛋白是如何聚合与解聚的？

艾美捷Actin聚合检测试剂盒的原理：

此肌动蛋白聚合分析生化试剂盒基于在聚合过程中pyrene（芘）结合Actin时荧光增强的原理。随着时间的推移，在聚合过程中，当芘G-actin（单体）形成芘F-actin（纤维形肌动蛋白）时能够检测到荧光增强。此外，通过使用预先形成的芘F-actin，还可以进行解聚。肌动蛋白聚合分析生化试剂盒是一种非常快速和经济的研究肌动蛋白聚合/解聚的方案。

艾美捷Actin聚合检测试剂盒的组分也可单独用于其他基于肌动蛋白的测定，例如检测F-肌动蛋白结合蛋白的旋转下降测定（也见BK001）或用于鉴定G-肌动素结合蛋白的大小排阻色谱。

艾美捷Actin聚合检测试剂盒化学性质：

中文名称：Actin聚合生化盒试剂

英文名：Actin Polymerization Biochem Kit

cat#：BK003

规格：KIT30-100次检测

保存建议：推荐常温运输，建议保存于4°C。

艾美捷Actin聚合检测试剂盒的应用：

1.通过添加组织提取物，肌动蛋白结合蛋白或化合物来分析其对Actin聚合或解聚的影响。

2.通过添加F-actin成核蛋白，化合物或提取物来分析其对Actin聚合的影响。

3.通过添加F-肌动蛋白切断蛋白，化合物或组织提取物来分析其对F-actin稳态水平的影响。

4.确定在各种实验条件下肌动蛋白聚合的临界浓度。