中英文说明书丨艾美捷细胞失巢凋亡检测试剂盒介绍

Assay Principle

Cells are cultured in poly-Hema coated plate or control plate. Cell viability is determined by MTT or

Calcein AM. Anoikis propelled cell death is measured by Ethidium Homodimer (EthD-1). EthD-1 is

an excellent marker for measuring dead cells. EthD-1 is a red fluorescent dye that can only penetrate

damaged cell membranes. EthD-1 will fluoresce with a 40-fold enhancement upon binding ssDNA,

dsDNA, RNA, oligonucleotides, and triplex DNA. Background fluorescence levels are very low

because the dyes are virtually non-fluorescent before interacting with cells.

Kit Components

1. Anchorage Resistant Plate (Part No. 108001): One 24-well Poly-Hema coated plate.

2. Calcein AM (500X) (Part No. 108002): One vial – 50 µL in DMSO.

3. Ethidium Homodimer (EthD-1) (500X) (Part No. 108003): One vial – 50 µL.

4. Detergent Solution (Part No. 108004): One bottle – 25.0 mL.

5. MTT Solution (Part No. 113502): Three tubes – 1.0 mL each.

Storage

Store the Calcein AM and Ethidium Homodimer at -20ºC. Store all other components at 4ºC.

艾美捷细胞失巢凋亡检测试剂盒提供比色和荧光格式测量锚定非依赖性生长并监测失巢细胞死亡。该套件包含足够的试剂用于在Poly-Hema涂层的24孔板中测定24个样品。活细胞是用MTT或Calcein AM检测。用埃塞俄比亚同二聚体（EthD-1）检测细胞死亡。细胞在聚Hema涂层板或对照板中培养。通过MTT或钙素AM。通过Ethidium Homodimer（EthD-1）测量失巢细胞死亡。EthD-1是一种用于测量死细胞的优良标记。EthD-1是一种红色荧光染料，只能穿透受损的细胞膜。EthD-1在结合ssDNA时将以40倍的增强发出荧光，dsDNA、RNA、寡核苷酸和三链DNA。背景荧光水平非常低因为染料在与细胞相互作用之前实际上是非荧光的。

  

艾美捷细胞失巢凋亡检测试剂盒组分：

1.抗锚固板（零件号108001）：一块24孔Poly-Hema涂层板。

2.钙黄素AM（500X）（零件号108002）：一小瓶–50µL DMSO。

3.埃塞俄比亚同二聚体（EthD-1）（500X）（零件号108003）：一小瓶–50µL。

4.洗涤剂溶液（零件号108004）：一瓶–25.0 mL。

5.MTT溶液（零件号113502）：三管，每管1.0 mL。

艾美捷细胞失巢凋亡检测试剂盒相关研究：

1. CBA-081: CytoSelect™ 96-Well Anoikis Assay

2. CBA-230: Cellular Senescence Detection Kit (SA-β-Gal Staining)

3. CBA-231: 96-Well Cellular Senescence Assay (SA β-Gal Activity)

4. CBA-232: Quantitative Cellular Senescence Assay (SA β-Gal)

5. CBA-240: CytoSelect™ Cell Viability and Cytotoxicity Assay

   

下图显示了CytoSelect的典型结果细胞失巢凋亡检测试剂盒。以下数据仅供参考。该数据不应用于解释实际后果。

图：人前胸成纤维细胞BJ-TERT细胞的失活测定。BJ-TERT细胞以50000个细胞/孔接种在组织培养对照板或Poly-Hema涂层板中。细胞是允许培养24小时。通过MTT和Calcein AM测定细胞活力用EthD-1染色失巢样细胞死亡。

细胞失巢凋亡检测试剂盒部分文献引用：

1. Lu, S. et al. (2016). Expression of α-fetoprotein in gastric cancer AGS cells contributes to invasion

and metastasis by influencing anoikis sensitivity. Oncol Rep. 35:2984-2990.

2. Lee, H.W. et al. (2013). Tpl2 kinase impacts tumor growth and metastasis of clear cell renal cell

carcinoma. Mol Cancer Res. 11:1375-1386.

3. Sisto, M. et al. (2009). Fibulin-6 expression and anoikis in human salivary gland epithelial cells:

implications in Sjogren's syndrome. Int. Immunol. 21:303-311.

4. Liu, H. et al. (2008). Cysteine-rich protein 61 and connective tissue growth factor induce deadhesion and anoikis of retinal pericytes. Endocrinology 149:1666-1677.